NMR-STUDIES OF MELANINS - CHARACTERIZATION OF A SOLUBLE MELANIN FREE ACID FROM SEPIA INK

被引：29

作者：

AIME, S ^{[1
]}

FASANO, M ^{[1
]}

TERRENO, E ^{[1
]}

GROOMBRIDGE, CJ ^{[1
]}

机构：

[1] UNIV LONDON ROYAL HOLLOWAY & BEDFORD NEW COLL,DEPT CHEM,EGHAM TX20 OEX,SURREY,ENGLAND

来源：

PIGMENT CELL RESEARCH | 1991年 / 4卷 / 5-6期

关键词：

CROSS POLARIZATION; MAGIC ANGLE SPINNING; MELANIN; NMR; PARAMAGNETIC RELAXATION;

D O I：

10.1111/j.1600-0749.1991.tb00443.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

This paper deals with the nuclear magnetic resonance characterization of a soluble derivative (melanin free acid) of Sepia melanin obtained by a peroxidative treatment of the parent (insoluble) species. High resolution C-13 and N-15 solid state NMR spectroscopies allow the assessment of the chemical changes occurring in the macromolecule upon solubilization. H-1 and C-13 NMR solution spectra are discussed in light of the results obtained from the solid state spectra. Furthermore, the coordination properties of melanin have been investigated through Al-27 NMR spectroscopy and proton relaxation enhancement studies of the paramagnetic gadolinium complex of melanin free acid. Through these experiments it has been possible to evaluate the molecular reorientational time-tau(R) (and from it an estimated molecular weight close to 20 KDa) and the strength of the metal-macromolecule interaction.

引用

页码：216 / 221

页数：6

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