CHEMOTAXIS AND CHEMOKINESIS OF HUMAN SPERMATOZOA TO FOLLICULAR FACTORS

被引:121
作者
RALT, D
MANOR, M
COHENDAYAG, A
TURKASPA, I
BENSHLOMO, I
MAKLER, A
YULI, I
DOR, J
BLUMBERG, S
MASHIACH, S
EISENBACH, M
机构
[1] WEIZMANN INST SCI,DEPT MEMBRANE RES & BIOPHYS,IL-76100 REHOVOT,ISRAEL
[2] TEL AVIV UNIV,SHEBA MED CTR,DEPT OBSTET & GYNECOL,IL-52621 TEL HASHOMER,ISRAEL
[3] TECHNION ISRAEL INST TECHNOL,RAMBAM MED CTR,FAC MED,INST INFERTIL,IL-32000 HAIFA,ISRAEL
[4] TEL AVIV UNIV,SACKLER FAC MED,SACKLER INST MOLEC MED,IL-69978 TEL AVIV,ISRAEL
关键词
D O I
10.1095/biolreprod50.4.774
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Human spermatozoa accumulate in vitro in diluted follicular fluids obtained from follicles from which the eggs have been fertilized. Using capillary assays under a variety of experimental conditions (ascending or descending gradients of follicular fluid, or no gradient at all) and microscopic assays in which individual spermatozoa could be followed, we found that the sperm accumulation in follicular fluid was the result of both sperm chemotaxis and chemokinesis and eventually hyperactivation-like motility, We determined the optimal conditions for sperm accumulation, which involved sperm preincubation (possibly to induce sperm capacitation) and proper dilution of follicular fluid. In all the assays, the not accumulation was low, probably reflecting the chemotactic responsiveness of only a small fraction of the sperm population at any given time. We partially fractionated follicular fluid in a Centricon microconcentrator (Amicon, Danvers, MA) and by acetone precipitation, and found that at least one of the chemotactic factors is a small (< 10-kDa) molecule that is probably nonhydrophobic. This is the first time that sperm chemotaxis and chemokinesis in response to a follicular factor(s) in mammals has been established and has been distinguished from other processes that might cause sperm accumulation. The physiological significance of these findings is discussed.
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收藏
页码:774 / 785
页数:12
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