3,4-DICHLOROISOCOUMARIN, A SERINE PROTEASE INHIBITOR, INACTIVATES GLYCOGEN PHOSPHORYLASE-B

被引：16

作者：

RUSBRIDGE, NM ^{[1
]}

BEYNON, RJ ^{[1
]}

机构：

[1] UNIV LIVERPOOL,DEPT BIOCHEM,PROTEOLYSIS RES GRP,POB 147,LIVERPOOL L69 3BX,ENGLAND

来源：

FEBS LETTERS | 1990年 / 268卷 / 01期

关键词：

3,4-Dichloroisocoumarin; Glycogen phosphorylase b;

D O I：

10.1016/0014-5793(90)80991-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

3,4-Dichloroisocoumarin (3,4-DCI) is a highly reactive, mechanism-based inhibitor of serine proteases. We show here that glycogen phosphorylase b is also inactivated by this inhibitor, in a mechanism that parallels the inactivation of serine proteases, but involving multiple sites of covalent modification. Such a process may compromise studies in which 3,4-DCI is used to arrest proteolysis of a second native protein which may itself be modified. © 1990.

引用

页码：133 / 136

页数：4

共 6 条

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