HEAT-INDUCED REVERSIBLE AND IRREVERSIBLE ALTERATIONS IN THE STRUCTURE OF PHASEOLUS-VULGARIS THYLAKOID PROTEINS

被引：9

作者：

ALKHATIB, K ^{[1
]}

WIEST, SC ^{[1
]}

机构：

[1] KANSAS STATE UNIV AGR & APPL SCI,DEPT HORT,WATERS HALL,MANHATTAN,KS 66506

来源：

JOURNAL OF THERMAL BIOLOGY | 1990年 / 15卷 / 3-4期

关键词：

Bean chlorophast; heat-stress; membrane protein structure; thylakoid membrane;

D O I：

10.1016/0306-4565(90)90008-6

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Chloroplast thylakoids isolated from bean (Phaseolus vulgaris L.) leaves have been used as a model system to identify heat-induced changes in membrane protein structure. Susceptibility of SDS gel electrophoresis bands to trypsin digestion was used as an assay for altered structure of proteins comprising the bands. Both reversible and irreversible alternations in membrane protein structure have been observed as a result of exposure to 35 or 45°C for varying time periods. © 1990.

引用

页码：239 / 244

页数：6

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