PCR DETECTION OF BORRELIA-BURGDORFERI DNA IN CEREBROSPINAL-FLUID OF LYME NEUROBORRELIOSIS PATIENTS

被引:101
作者
KELLER, TL
HALPERIN, JJ
WHITMAN, M
机构
[1] HARVARD UNIV, SCH MED, DANA FARBER CANC INST, INST CELL & MOLEC BIOL, BOSTON, MA 02115 USA
[2] SUNY STONY BROOK, DEPT NEUROL, STONY BROOK, NY 11794 USA
[3] HARVARD UNIV, DEPT BIOCHEM & MOLEC BIOL, CAMBRIDGE, MA 02138 USA
[4] HARVARD UNIV, SCH MED, DEPT CELL & MOLEC PHYSIOL, BOSTON, MA 02115 USA
关键词
D O I
10.1212/WNL.42.1.32
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
We used the polymerase chain reaction (PCR), a method useful in the detection of Borrelia burgdorferi in vitro, to evaluate CSF in patients thought to have neuroborreliosis. Nested pairs of oligonucleotide primers were designed to recognize the C-terminal region of B burgdorferi OspA. CSF samples were obtained from (1) patients with immunologic evidence of systemic B burgdorferi infection and clinical manifestations suggestive of CNS dysfunction, (2) seronegative patients with clinical disorders consistent with Lyme borreliosis, and (3) patient and contamination controls; all were analyzed in a blinded fashion. PCR detected B burgdorferi OspA DNA in CSF of (1) 10 of 11 patients with Lyme encephalopathy, (2) 28 of 37 patients with inflammatory CNS disease, (3) seven of seven seronegative patients with Lyme-compatible disorders, and (4) zero of 23 patient controls. Zero of 83 additional contamination controls were PCR-positive. In eight patients from whom we obtained CSF before and after parenteral antimicrobial therapy, PCR results invariably predicted clinical outcome accurately.
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页码:32 / 42
页数:11
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