DETECTION OF ALKOXYL AND CARBON-CENTERED FREE-RADICALS IN CORONARY SINUS BLOOD FROM PATIENTS UNDERGOING ELECTIVE CARDIOPLEGIA

被引:87
作者
TORTOLANI, AJ
POWELL, SR
MISIK, V
WEGLICKI, WB
POGO, GJ
KRAMER, JH
机构
[1] GEORGE WASHINGTON UNIV, MED CTR,DEPT MED,DIV EXPTL MED,ROOM 407A, ROSS HALL,2300 EYE ST NW, WASHINGTON, DC 20037 USA
[2] N SHORE UNIV HOSP, DEPT SURG, MANHASSET, NY 11030 USA
[3] CORNELL UNIV, COLL MED, MANHASSET, NY 11030 USA
[4] GEORGE WASHINGTON UNIV, MED CTR, DEPT PHYSIOL, DIV EXPTL MED, WASHINGTON, DC 20037 USA
关键词
CARDIOPULMONARY BYPASS; ALPHA-PHENYL-TERT-BUTYLNITRONE; ISCHEMIA; SPIN TRAPPING; FREE RADICALS;
D O I
10.1016/0891-5849(93)90091-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Confirmation of the involvement of free radicals in postischemic injury in human heart has been elusive. The present study was performed to determine the presence of free radicals in coronary sinus blood from patients undergoing elective open heart surgery and cardioplegia. Six patients who were scheduled for nonurgent elective open heart surgery were used in this study. Coronary sinus blood samples were withdrawn at 1, 3, 5, 10, 15, 20, and 25 min post-cross-clamp and immediately mixed with isosmotic alpha-phenyl-tert-butylnitrone (PBN) and then centrifuged to obtain plasma. Plasma samples were extracted with toluene and analyzed using electron spin resonance (ESR) spectroscopy. We observed ESR spectra consistent with the formation of alkoxyl and carbon-centered radical adducts of PBN (a(N) = 13.6 G, a(beta)H = 1.9 G, and a(N) = 14.1 G, a(beta)H = 4.2 G) in six of six patients. We obtained complete free radical production time courses during reperfusion from five patients, and all demonstrated a biphasic profile with an initial burst from 5 to 10 min followed by a second maxima at 25 min. Total PBN-adduct production during reperfusion increased in patients subjected to longer aortic cross-clamp times (global ischemia). These data demonstrate that postcardioplegia free radical production is detectable in coronary sinus blood using an ex vivo spin-trapping technique and that the extent of formation may be related to the severity of ischemia.
引用
收藏
页码:421 / 426
页数:6
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