MICROPURIFICATION AND AMINO-ACID-SEQUENCE OF BETA-CASOMORPHIN-8 IN MILK FROM A WOMAN WITH POSTPARTUM PSYCHOSIS

被引：17

作者：

RENLUND, S

ERLANDSSON, I

HELLMAN, U

SILBERRING, J

WERNSTEDT, C

LINDSTROM, L

NYBERG, F

机构：

[1] UPPSALA UNIV, DEPT PHARMACOL, S-75124 UPPSALA, SWEDEN

[2] LUDWIG INST CANC RES, UPPSALA BRANCH, S-75124 UPPSALA, SWEDEN

[3] UPPSALA UNIV, VASTERAS HOSP, PSYCHIAT RES UNIT, S-72189 VASTERAS, SWEDEN

[4] KAROLINSKA INST, DEPT DRUG DEPENDENCE RES, S-10401 STOCKHOLM 60, SWEDEN

来源：

PEPTIDES | 1993年 / 14卷 / 06期

关键词：

MICROPURIFICATION; HPLC; HUMAN; MILK; POSTPARTUM PSYCHOSIS; BETA-CASOMORPHIN; BETA-CASOMORPHIN-8; IMMUNOREACTIVE; RADIOIMMUNOASSAY; GAS PHASE AMINO ACID SEQUENCE ANALYSIS; SOLID PHASE AMINO ACID SEQUENCE ANALYSIS; FAST ATOM BOMBARDMENT MASS-SPECTROMETRY;

D O I：

10.1016/0196-9781(93)90165-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Milk was obtained from a woman with acute postpartum psychosis and with ongoing lactation. Defatted samples were subjected to micropurification and collected fractions were analyzed by means of their beta-casomorphin-8 immunoreactivity. Immunoreactive material with the same chromatographic properties as synthetic human beta-casomorphin-8 was determined by amino acid sequence analysis to be Tyr-Pro-Phe-Val-Glu-Pro-Ile-Pro. Its molecular mass was determined by fast atom bombardment-mass spectrometry to be 962.3 Da. These determinations, which ultimately identify the immunoreactive material as human beta-casomorphin-8, represent the first structural identification of a beta-casomorphin peptide from a body fluid.

引用

页码：1125 / 1132

页数：8

共 40 条

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