A PLATE ASSAY FOR THE DETECTION OF ORGANOPHOSPHONATE MINERALIZATION BY ENVIRONMENTAL BACTERIA, AND ITS MODIFICATION AS AN ACTIVITY STAIN FOR IDENTIFICATION OF THE CARBON-PHOSPHORUS BOND-CLEAVAGE ENZYME PHOSPHONOACETATE HYDROLASE

被引：5

作者：

MCGRATH, JW ^{[1
]}

QUINN, JP ^{[1
]}

机构：

[1] QUEENS UNIV BELFAST,CTR MED BIOL,CTR QUESTOR,BELFAST BT9 7BL,ANTRIM,NORTH IRELAND

来源：

BIOTECHNOLOGY TECHNIQUES | 1995年 / 9卷 / 07期

关键词：

D O I：

10.1007/BF00159565

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A replica plate screening technique, based on the acid molybdate assay for detection of phosphate has been developed to permit the detection of microorganisms capable of mineralizing organophosphonates. The method was further adapted as the basis of an activity stain for the detection of the carbon - phosphorus bond cleavage enzyme phosphonoacetate hydrolase in PAGE gels.

引用

页码：497 / 502

页数：6

共 8 条

[1]

Bartha R., 1990, Isolation of biotechnogical organisms from nature., P283

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3] DISC ELECTROPHORESIS .2. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS [J].

DAVIS, BJ .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1964, 121 (A2) :404-&

[4]

Fiske CH, 1925, J BIOL CHEM, V66, P375

[5]

Hilderbrand R.L., 1983, P5

[6] METABOLISM OF PHOSPHONOACETATE AS THE SOLE CARBON AND PHOSPHORUS SOURCE BY AN ENVIRONMENTAL BACTERIAL ISOLATE [J].

MCMULLAN, G ;

HARRINGTON, F ;

QUINN, JP .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (04) :1364-1366

[7] IN-VITRO CHARACTERIZATION OF A PHOSPHATE STARVATION-INDEPENDENT CARBON-PHOSPHORUS BOND-CLEAVAGE ACTIVITY IN PSEUDOMONAS-FLUORESCENS 23F [J].

MCMULLAN, G ;

QUINN, JP .

JOURNAL OF BACTERIOLOGY, 1994, 176 (02) :320-324

[8] OXIDATION OF ARSENITE TO ARSENATE BY ALCALIGENES-FAECALIS [J].

PHILLIPS, SE ;

TAYLOR, ML .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1976, 32 (03) :392-399

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