RAPID IDENTIFICATION OF RHIZOBIUM SPECIES BASED ON CELLULAR FATTY-ACID ANALYSIS

被引:42
作者
JARVIS, BDW [1 ]
TIGHE, SW [1 ]
机构
[1] ANALYT SERV INC,ESSEX JCT,VT 05453
关键词
FAME; FATTY ACID ANALYSIS; RAPID IDENTIFICATION; RHIZOBIUM;
D O I
10.1007/BF02183083
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
As understanding of the evolutionary relationships between strains and species of root nodule bacteria increases the need for a rapid identification method that correlates well with phylogenetic relationships is clear. We have examined 123 strains of Rhizobium: R. fredii (19), R. galegae (20), R. leguminosarum (22), R. loti (17), R. meliloti (21), and R. tropici (18) and six unknowns. All strains were grown on modified tryptone yeast-extract (TY) agar, as log phase cultures, scraped from the agar, lysed, and the released fatty acids derivatized to their corresponding methyl esters. The methyl esters were analysed by gas-chromatography using the MIDI/Hewlett-Packard Microbial Identification System. All species studied contained 16:0, 17:0, 18:0 and 19cyclo(w) 9C fatty acids but only R loti and R tropici produced 12:0 3 OH,13:0 iso 3 OH,18:1(w)9C and 15:0 iso 3 OH,17.0 iso 3 OH and 20:2(w)6,9C fatty acids respectively. Principal component analysis was used to show that strains could be divided into clusters corresponding to the six species. Fatty acid profiles for each species were developed and these correctly identified at least 95% of the strains belonging to each species. A dendrogram is presented showing the relationships between Rhizobium species based on fatty acid composition. The data base was used to identify unknown soil isolates as strains of Rhizobium lacking a symbiotic plasmid and a bacterium capable of expressing a symbiotic plasmid from R. leguminosarum as Sphingobacterium spiritovorum.
引用
收藏
页码:31 / 41
页数:11
相关论文
共 16 条
  • [1] BERINGER JE, 1974, J GEN MICROBIOL, V84, P188
  • [2] RHIZOBIUM-HUAKUII SP-NOV ISOLATED FROM THE ROOT-NODULES OF ASTRAGALUS-SINICUS
    CHEN, WX
    LI, GS
    QI, YL
    WANG, ET
    YUAN, HL
    LI, JL
    [J]. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1991, 41 (02): : 275 - 280
  • [3] Dangeard P.A., 1926, BOTANISTE, V16, P1
  • [4] Frank B, 1879, BOT ZTG, V37, P376
  • [5] Frank B., 1879, BOT ZTG, V37, P394
  • [6] PROPOSED MINIMAL STANDARDS FOR THE DESCRIPTION OF NEW GENERA AND SPECIES OF ROOT-NODULATING AND STEM-NODULATING BACTERIA
    GRAHAM, PH
    SADOWSKY, MJ
    KEYSER, HH
    BARNET, YM
    BRADLEY, RS
    COOPER, JE
    DELEY, DJ
    JARVIS, BDW
    ROSLYCKY, EB
    STRIJDOM, BW
    YOUNG, JPW
    [J]. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1991, 41 (04): : 582 - 587
  • [7] RHIZOBIUM-LOTI, A NEW SPECIES OF LEGUME ROOT NODULE BACTERIA
    JARVIS, BDW
    PANKHURST, CE
    PATEL, JJ
    [J]. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1982, 32 (03): : 378 - 380
  • [8] PHYLOGENY OF FAST-GROWING SOYBEAN-NODULATING RHIZOBIA SUPPORTS SYNONYMY OF SINORHIZOBIUM AND RHIZOBIUM AND ASSIGNMENT TO RHIZOBIUM-FREDII
    JARVIS, BDW
    DOWNER, HL
    YOUNG, JPW
    [J]. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1992, 42 (01): : 93 - 96
  • [9] INTRAGENERIC AND INTERGENERIC SIMILARITIES BETWEEN THE RIBOSOMAL RIBONUCLEIC-ACID CISTRONS OF RHIZOBIUM AND BRADYRHIZOBIUM SPECIES AND SOME RELATED BACTERIA
    JARVIS, BDW
    GILLIS, M
    DELEY, J
    [J]. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1986, 36 (02): : 129 - 138
  • [10] RHIZOBIUM-GALEGAE, A NEW SPECIES OF LEGUME ROOT NODULE BACTERIA
    LINDSTROM, K
    [J]. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1989, 39 (03): : 365 - 367