CULTURAL AND MORPHOLOGICAL-CHARACTERISTICS OF HUMAN PERIODONTAL-LIGAMENT CELLS IN-VITRO

被引：16

作者：

ADAMS, AM ^{[1
]}

SOAMES, JV ^{[1
]}

SEARLE, RF ^{[1
]}

机构：

[1] UNIV NEWCASTLE UPON TYNE,SCH MED,DEPT IMMUNOL,NEWCASTLE TYNE NE2 4BW,ENGLAND

来源：

ARCHIVES OF ORAL BIOLOGY | 1993年 / 38卷 / 08期

关键词：

CELL CULTURE; ELECTRON MICROSCOPY; HUMAN PERIODONTAL LIGAMENT;

D O I：

10.1016/0003-9969(93)90004-6

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Primary cell lines from explants of human periodontal ligament were established in vitro to assess their suitability as test cells in a biocompatibility assay. They were maintained over nine passages. The constituent fibroblast-like cells (F-cells) were monitored throughout by light and electron microscopy. Although F-cells from several different donors displayed stable and consistent growth characteristics, after the ninth subculture changes in the fine structure suggested that some cells were undergoing differentiation or senescence. It is therefore recommended that cells from earlier passages be used in in vitro biocompatibility assays.

引用

页码：657 / 662

页数：6

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