EXPRESSION OF 5-LIPOXYGENASE IN LYMPHOBLASTOID B-CELLS AND T-CELLS

被引：16

作者：

ELMAKHOURHOJEIJ, Y

BACLET, MC

CHABLERABINOVITCH, H

BENEYTOUT, JL

COOK, J

机构：

[1] U.R.A. CNRS 1485, Faculté de Médecine

来源：

PROSTAGLANDINS | 1994年 / 48卷 / 01期

关键词：

5-LIPOXYGENASE; 5-LIPOXYGENASE ACTIVATING PROTEIN; LYMPHOCYTES; SKW6.4; CESS; MOLT4;

D O I：

10.1016/0090-6980(94)90093-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The expression of arachidonate 5-lipoxygenase (5-LOX, arachidonate: oxygen 5-ixudiredyctase; EC 1.13.11.34) and the 5-lipoxygenase activating protein (FLAP) genes in lymphoblastoid B and T cell lines was studied at the transcriptional level by reverse transcription-PCR analysis. Two B cell lines, CESS and SKW 6.4, were found to express both 5-LOX and FLAP mRNA. One T cell line, MOLT 4, expressed only FLAP mRNA. Upon stimulation of intact cells by calcium ionophore, B lymphoblastoid cells produced 5, 12 and 15 hydroxyeicosatetraenoic acids as well as leukotriene B-4.

引用

页码：21 / 29

页数：9

共 8 条

[1] 5-LIPOXYGENASE-ACTIVATING PROTEIN STIMULATES THE UTILIZATION OF ARACHIDONIC-ACID BY 5-LIPOXYGENASE
ABRAMOVITZ, M
WONG, E
COX, ME
RICHARDSON, CD
LI, C
VICKERS, PJ
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1993, 215 (01): : 105 - 111
[2] BORGEAT P, 1984, J ALLERGY CLIN IMMUN, V74, P310, DOI 10.1016/0091-6749(84)90122-2
[3] CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[4] FUNK CD, 1991, J BIOL CHEM, V266, P12508
[5] ON THE EXPRESSION AND REGULATION OF 5-LIPOXYGENASE IN HUMAN-LYMPHOCYTES
JAKOBSSON, PJ
STEINHILBER, D
ODLANDER, B
RADMARK, O
CLAESSON, HE
SAMUELSSON, B
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (08) : 3521 - 3525
[6] ODLANDER B, 1989, INT J TISSUE REACT, V11, P277
[7] SCHULAM PG, 1990, J IMMUNOL, V144, P2696
[8] YAMAOKA KA, 1989, J IMMUNOL, V143, P1996

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