NEUROPEPTIDES AND SEROTONIN IN THE CESTODE, PROTEOCEPHALUS-EXIGUUS - AN IMMUNOCYTOCHEMICAL STUDY

被引:22
作者
GUSTAFSSON, MKS
FAGERHOLM, HP
HALTON, DW
HANZELOVA, V
MAULE, AG
REUTER, M
SHAW, C
机构
[1] QUEENS UNIV BELFAST, COMPARAT NEUROENDOCRINOL RES GRP, BELFAST BT7 1NN, ANTRIM, NORTH IRELAND
[2] SLOVAK ACAD SCI, INST PARASITOL, KOSICE 04001, SLOVAKIA
关键词
PROTEOCEPHALUS EXIGUUS; CESTODE; NERVOUS SYSTEM; NEUROPEPTIDE F; RFAMIDE; 5-HT; IMMUNOCYTOCHEMISTRY; CONFOCAL SCANNING LASER MICROSCOPY;
D O I
10.1016/0020-7519(94)00169-O
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Neuropeptide F (NPF), RFamide and serotonin (5-HT) immunoreactivities have been detected in the nervous system of P. exiguus procercoids and adults, using an indirect immunocytochemical technique in conjunction with confocal scanning laser microscopy. The peptidergic nervous system of the procercoid is well developed, with two brain ganglia, three pairs of longitudinal nerve cords, transverse ring commissures and nerves in the suckers, all showing NPF-immunostaining. Strong NPF- and RF-immunostaining was observed in the CNS and PNS of the adult worm. The distribution patterns of the two neuropeptides were similar. Immunoreactivity for 5-HT was found only in the CNS.
引用
收藏
页码:673 / 682
页数:10
相关论文
共 24 条
  • [1] Coons, Leduc, Connolly, Studies on antibody production. I. A method of the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit, Journal of Experimental Medicine, 102, pp. 49-60, (1955)
  • [2] Fairweather, MaCartney, Johnston, Halton, Buchanan, Immunocytochemical demonstration of 5-hydroxytryptamine (serotonin) and vertebrate neuropeptides in the nervous system of excysted cysticercoid larvae of the rat tapeworm, Hymenolepis diminuta, Parasitology Research, 74, pp. 371-379, (1988)
  • [3] Fairweather, Mahendrasingam, Johnston, Halton, McCullough, Shaw, An ontogenetic study of the cholinergic and serotoninergic nervous systems in Trilocularia acanthiaevulgaris (Cestoda, Tetraphyllidea), Parasitology Research, 76, pp. 487-496, (1990)
  • [4] Fairweather, Mahendrasingam, Johnston, Halton, Shaw, Peptidergic nerve elements in three developmental stages of the tetraphyllidean tapeworm Trilocularia acanthiaevulgaris. An immunocytochemical study, Parasitology Research, 76, pp. 497-508, (1990)
  • [5] Freeman, Ontogeny of cestodes and its behaviour on their phylogeny and systematics, Advances in Parasitology, 11, pp. 481-557, (1973)
  • [6] Greenberg, Price, Invertebrate neuropeptides: native and naturalized, Annual Review of Physiology, 45, pp. 271-288, (1983)
  • [7] Grimmelikhuijzen, Antisera to the sequence Arg-Phe amide visualize neuronal centralization in hydroid pylops, Cell and Tissue Research, 241, pp. 171-182, (1985)
  • [8] Gustafsson, Skin the tapeworms before you stain their nervous system! A new method for whole-mount immunocytochemistry, Parasitology Research, 77, pp. 509-516, (1991)
  • [9] Gustafsson, The neuroanatomy of parasitic flatworms, Advances in Neuroimmunology, 2, pp. 267-286, (1992)
  • [10] Gustafsson, Halton, Maule, Reuter, Shaw, The gull-tapeworm Diphyllobothrium dendriticum and neuropeptide F: an immunocytochemical study, Parasitology, 109, pp. 599-609, (1994)