EVALUATION OF ASSAYS FOR THE IDENTIFICATION AND QUANTITATION OF MUCONIC ACID, A BENZENE METABOLITE IN HUMAN URINE

被引:27
作者
BARTCZAK, A
KLINE, SA
YU, R
WEISEL, CP
GOLDSTEIN, BD
WITZ, G
BECHTOLD, WE
机构
[1] ENVIRONM & OCCUPAT HLTH SCI INST,DIV TOXICOL,PISCATAWAY,NJ 08855
[2] UNIV MED & DENT NEW JERSEY,ROBERT WOOD JOHNSON MED SCH,DEPT ENVIRONM & COMMUNITY MED,PISCATAWAY,NJ 08854
[3] LOVELACE BIOMED & ENVIRONM RES INST,INHALAT TOXICOL RES INST,ALBUQUERQUE,NM 87185
来源
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH | 1994年 / 42卷 / 03期
关键词
D O I
10.1080/15287399409531877
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Muconic acid (MA) is a urinary metabolite of benzene and has been used as a biomarker of exposure to benzene in humans exposed to levels as low as 1 ppm. We have modified a high-pressure liquid chromatography (HPLC) based assay for urinary MA (Ducos et al., 1990) by the use of a diode array detector. This modification increases the specificity of the HPLC-based assay by identifying raise positives. In addition, we have developed a gas chromatography (GC) based assay that uses a flame ionization detector (GC-FID). Both assays identified and quantified MA in human urine at concentrations greater than 40-50 ng/ml. Assay precision was within 10% relative standard deviation for MA concentrations above 90 ng/ml using the HPLC assay and above 40 ng/ml using the CC-FID assay. Quantitative accuracy of the assays was evaluated by determining MA in human urine samples using both methods and also a gas chromatography-mass spectrometry (GC-MS) procedure. Numerical correlation among the three assays was good at MA concentrations above 100 ng/ml.
引用
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页码:245 / 258
页数:14
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