ULTRAVIOLET LIGHT-INDUCED DNA DAMAGE IN TRANSCRIBED SEQUENCES - NO CHANGE IN REPAIR WITH AGE

被引：6

作者：

KUNISADA, T ^{[1
]}

MILLER, CD ^{[1
]}

SCHNEIDER, EL ^{[1
]}

机构：

[1] NIA,GERONTOL RES CTR,MOLEC GENET LAB,BALTIMORE,MD 21224

来源：

MUTATION RESEARCH | 1990年 / 237卷 / 02期

关键词：

CAT gene; Plasmid vector; UV light;

D O I：

10.1016/0921-8734(90)90013-H

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We studied the repair of a plasmid vector containing the chloramhenicol acetyltransferase (CAT) gene by treating the plasmid with UV light and then transfecting this plasmid into fibroblasts from human fetal lung (in vitro aging) and into primary cultured fibroblasts from rat lung and skin. This methodology allows us to examine the repair of specific transcribed DNA sequences. There was no age-related change in the repair of UV damage in these cells. Rat embryo fibroblasts at different passages transfected with the plasmid also revealed no significant alteration in UV repair as a function of passage number. © 1990.

引用

页码：75 / 81

页数：7

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