RADIOIMMUNOASSAY FOR ALBUTEROL USING A MONOCLONAL-ANTIBODY - APPLICATION FOR DIRECT QUANTIFICATION IN HORSE URINE

被引:28
作者
ADAM, A
ONG, H
SONDAG, D
RAPAILLE, A
MARLEAU, S
BELLEMARE, M
RAYMOND, P
GIROUX, D
LOO, JK
BEAULIEU, N
机构
[1] STATE UNIV LIEGE,B-4000 SART,BELGIUM
[2] UNIV MONTREAL,FAC MED VET,ST HYACINTHE J2S 7C6,QUEBEC,CANADA
[3] DIV PHARMACEUT CHEM,HLTH PROTECT BRANCH,OTTAWA K1A OL2,ONTARIO,CANADA
来源
JOURNAL OF IMMUNOASSAY | 1990年 / 11卷 / 03期
关键词
albuterol; clentuberol; monoclonal antibody; radioimmunoassay;
D O I
10.1080/01971529008055036
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A monoclonal antibody was synthesized in mouse against the 0-(3-carboxypropionyl) derivative of albuterol linked to bovine serum albumin. Isotyping of this material revealed the IgGl class characterized by an affinity constant of 1.03 nM"1 and a density of sites of 0.55 nM. This antibody was found specific as its cross-reactivity to structurally related molecules was less than 1% except for clenbuterol (75%). A radioimmunoassay was set up with culture supernatant (final dilution 1/1000) and [ H] albuterol. The calibration curve was characterized by a maximum binding of 28%, an ED50 of 1.15 pmol per tube, the detection 1imit was 28.8 fmol/tube and the linearity of the response was up to 39.8 pmol/tube. This RIA method has been used for direct quantitation of albuterol in horse urine without any clean-up or extration step. © 1990, Taylor & Francis Group, LLC. All rights reserved.
引用
收藏
页码:329 / 345
页数:17
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