TRANS-STIMULATION EFFECT ON H+-ORGANIC CATION ANTIPORT SYSTEM IN RAT RENAL BRUSH-BORDER MEMBRANES

被引：18

作者：

KATSURA, T ^{[1
]}

MAEGAWA, H ^{[1
]}

TOMITA, Y ^{[1
]}

TAKANO, M ^{[1
]}

INUI, KI ^{[1
]}

HORI, R ^{[1
]}

机构：

[1] KYOTO UNIV HOSP,FAC MED,DEPT PHARM,KYOTO 606,JAPAN

来源：

AMERICAN JOURNAL OF PHYSIOLOGY | 1991年 / 261卷 / 05期

关键词：

TETRAETHYLAMMONIUM TRANSPORT; PH SENSITIVITY; RENAL SECRETION; RENAL TUBULE;

D O I：

10.1152/ajprenal.1991.261.5.F774

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Trans-stimulation effect on tetraethylammonium (an organic cation) transport was examined in rat renal brush-border membrane vesicles. The uptake of [C-14]tetraethylammonium at pH 6.0-8.5 was stimulated by preloading the membrane vesicles with unlabeled tetraethylammonium. When the uptake was measured in preloaded membrane vesicles in the presence of carbonyl cyanide p-trifluoromethoxyphenylhydrazone, stimulation was observed at high pH but not at low pH. These results suggest that the mechanisms of the trans-stimulation effect on organic cation transport are different depending on the pH. When pH is low, the trans-stimulation is due to generation of an outward H+ gradient, which in turn stimulates [C-14]tetraethylammonium uptake by H+-[C-14]tetraethylammonium exchange. In contrast, when pH is high, the stimulation is due to direct exchange of tetraethylammonium for [C-14]tetraethylammonium.

引用

页码：F774 / F778

页数：5

共 34 条

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