INVITRO INVESTIGATIONS WITH FOSFOMYCIN ON MUELLER-HINTON AGAR WITH AND WITHOUT GLUCOSE-6-PHOSPHATE

Determination of the minimal inhibitory concentration of fosfomycin in the agar dilution test on Mueller-Hinton agar showed that the addition of glucose-6-phosphate to the nutrient medium potentiates the action of fosfomycin against Escherichia coli, Klebsiella, Enterobacter, Citrobacter and Staphylococcus aureus, sometimes by as much as 256-fold. Such a potentiation of action was not detectable with Serratia marcescens, the individual Proteus species, Pseudomonas aeruginosa or enterococci. Fosfomycin is very effective against most medically important bacterial species on Mueller-Hinton agar containing 25 μg/ml glucose-6-phosphate. Over 90% of the cultures of E. coli, Citrobacter, Enterobacter, S. marcescens, Proteus mirabilis, Proteus vulgaris, Proteus rettgeri, P. aeruginosa, S. aureus and enterococci examined were inhibited by ≤ 64 μg/ml fosfomycin. © 1979 Verlagsgesellschaft Otto Spatz.