MOLECULAR-CLONING OF A MOUSE EXTRACELLULAR SIGNAL REGULATED KINASE (ERK-1)

被引：3

作者：

TANNER, B ^{[1
]}

MUECKLER, M ^{[1
]}

机构：

[1] WASHINGTON UNIV,SCH MED,DEPT CELL BIOL & PHYSIOL,660 S EUCLIN,BOX 8228,ST LOUIS,MO 63110

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1171卷 / 03期

关键词：

EXTRACELLULAR SIGNAL REGULATED KINASE; ERK-1; CDNA;

D O I：

10.1016/0167-4781(93)90074-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A complementary DNA has been cloned and sequenced for the mouse extracellular signal regulated kinase (erk-1). The nucleotide sequence of the 1.7 kb mouse erk-1 cDNA clone (merk-1-34) is 93.5% identical to the published rat DNA sequence (Boulton et al. (1990) Science 249, 64-67). The mouse clone, as with all other reported sequences, did not contain an in-frame translation initiation codon and, therefore, does not appear to be full-length. Attempts to generate cDNA sequence upstream of the 5' end of merk-1-34 using thermostable reverse transcriptase and chemical melting of the mRNA (methylmercury hydroxide) were unsuccessful.

引用

页码：319 / 320

页数：2

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