KERATAN SULFATE INHIBITS ITS RELEASE IN RABBIT CHONDROCYTE

被引：5

作者：

FUKUDA, K

OHTANI, K

MATSUMURA, F

TANAKA, S

机构：

[1] Department of Orthopaedic Surgery, Kinki University School of Medicine, Osakasayama, Osaka

来源：

CONNECTIVE TISSUE RESEARCH | 1993年 / 30卷 / 01期

关键词：

PROTEOGLYCANS; SYNTHESIS; REGULATION CHONDROCYTES; KERATAN SULFATE;

D O I：

10.3109/03008209309032931

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Keratan sulfate (KS) is one of the major glycosaminoglycans in cartilage matrix proteoglycan. We find that exogenously added KS totally blocks keratanase sensitive glycosaminoglycan release measured by radiolabeled ligand. We also find that KS release is potentially inhibited by its own presence as measured by ELISA. When KS is digested with keratanase before its addition to the medium, its inhibitory effect on KS secretion is partially blocked. Exogenously added KS promotes the accumulation of the KS epitope in the cell layer. These data suggest that KS negatively regulates its own secretion.

引用

页码：75 / 83

页数：9

共 25 条

[1] PROGENY OF RABBIT ARTICULAR CHONDROCYTES SYNTHESIZE COLLAGEN TYPE-1 AND TYPE-3 AND TYPE-1 TRIMER, BUT NOT TYPE-2 - VERIFICATIONS BY CYANOGEN-BROMIDE PEPTIDE ANALYSIS [J].