CLONING AND EXPRESSION IN ESCHERICHIA-COLI OF THE DNAK GENE OF ZYMOMONAS-MOBILIS

被引：18

作者：

MICHEL, GPF

机构：

来源：

JOURNAL OF BACTERIOLOGY | 1993年 / 175卷 / 10期

关键词：

D O I：

10.1128/JB.175.10.3228-3231.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The DnaK protein of Zymomonas mobilis (DnaKz) was identified and found to be 80% identical to the DnaK protein of Escherichia coli on the basis of the sequence of the N-terminal 21 amino acids. The dnaKz gene was cloned and found to be expressed in a thermosensitive dnaK mutant of Escherichia coli. Expression of the foreign gene restored a thermoresistant phenotype but failed to modulate the heat shock response in E. coli.

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页码：3228 / 3231

页数：4

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