A SECRETED MUCIN CARRYING SIALYL-LEWIS A FROM COLON-CARCINOMA CELLS BINDS TO E-SELECTIN AND INHIBITS HL-60 CELL-ADHESION

被引：29

作者：

ZHANG, K ^{[1
]}

BAECKSTROM, D ^{[1
]}

HANSSON, GC ^{[1
]}

机构：

[1] GOTHENBURG UNIV,DEPT MED BIOCHEM,S-41390 GOTHENBURG,SWEDEN

来源：

INTERNATIONAL JOURNAL OF CANCER | 1994年 / 59卷 / 06期

关键词：

D O I：

10.1002/ijc.2910590619

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Sialyl-Lewis x and a are known as ligands for E-selectin (ELAM-I) involved in leukocyte-endothelial adhesion. L-CanAg (light cancer antigen), secreted by a colon carcinoma cell line COLO 205, is a soluble mucin-type glycoprotein expressing sialyl-Lewis a antigens. L-CanAg was purified from spent culture medium by trichloracetic acid precipitation and Superose 6 gel filtration. With a monoclonal antibody against E-selectin (BBAI) as a positive control, the purified L-CanAg was shown to bind to E-selectin-Fc coated into plastic microtiter wells and to the surface of transiently E-selectin-transfected COS-I cells in a Ca2+-dependent way. Immunofluorescent double labelling showed that both BBAI and L-CanAg stained the same cells and morphological co-localization on E-selectin-transfeeted COS-I cells. Like BBAI, L-CanAg can inhibit leukocyte HL-60 cell adhesion to E-selectin-transfected COS-I cells, and this inhibition can be blocked by a F(ab')(2) fragment directed against the sialyl-Lewis a epitope. (C) 1994 Wiley-Liss, Inc.

引用

页码：823 / 829

页数：7

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