GROWTH-POTENTIAL OF INDIVIDUAL STRAINS OF LISTERIA-MONOCYTOGENES IN FRESH VACUUM-PACKAGED REFRIGERATED GROUND TOP ROUNDS OF BEEF

Separate inocula of four strains of Listeria monocytogenes were prepared at 4 degrees C and inoculated (3.58 to 4.67 log(10) colony forming units [CFU]/g) in top round ground beef (< 4.0% fat) patties (78.8 +/- 6.7 g) of normal (5.47 +/- 0.03) or high (6.14 +/- 0.08) pH, which were stored (4 degrees C) vacuum-packaged for 56 days and analyzed for L. monocytogenes, total aerobic plate counts (APC) and pH. In normal-pH ground beef, strain N-7143 (serotype 3a), multiplied from 4.25 +/- 0.71 log(10) CFU/g at day of inoculation to 6.53 +/- 0.34 log(10) CFU/g at 35 days of storage (P < 0.05); a 2.3 log(10) CFU/g increase. Populations of strain Na-19 (serotype 3b) increased 1.8 log(10) CFU/g in 35 days of storage, while numbers of strain Na-16 (serotype 1/2a) did not change (P > 0.05) during the 56 days of storage. Strain Scott A (serotype 4b) decreased in numbers from 4.00 +/- 1.21 log(10) CFU/g at day-0 to 2.72 +/- 0.98 log(10) CFU/g at 56 days. Populations of strain Scott A were lower (P < 0.05) than other strains after 21 days of storage. In high-pH ground beef, populations of strains Na-19, N-7143 and Na-16 increased (P < 0.05) by 2.87, 2.64 and 2.24 log(10) CFU/g, respectively, in 28 days. Populations of strain Scott A did not change significantly (P > 0.05), and they were lower (P < 0.05) than populations of other strains at 28 days. The results indicated that although growth of L. monocytogenes in vacuum-packaged, refrigerated ground beef was slow, it proceeded more rapidly in product of pH values above 6.0, and depended on strain of the pathogen tested.