PHYSICAL MAPPING OF A PLASMID FROM BACILLUS-MEGATERIUM BY RESTRICTION ENDONUCLEASE CLEAVAGE

A 4-Mdalton plasmid from Bacillus megaterium strain 216 has been physically mapped by restriction endonuclease digestion. A combination of single and double digests with seven restriction enzymes, together with a terminal labeling procedure, has produced a physical map containing 21 apparently unique cleavage sites. The data are most consistent with the view that this plasmid does not contain extensive variability in sequence. © 1979.