EXPRESSION OF GENES FOR THE MYELIN-SPECIFIC PROTEINS IN OLIGODENDROCYTES INVIVO DEMANDS THE PRESENCE OF AXONS

被引：24

作者：

GOTO, K

KURIHARA, T

TAKAHASHI, Y

KONDO, H

机构：

[1] NIIGATA UNIV,BRAIN RES INST,DEPT NEUROPHARMACOL,NIIGATA 951,JAPAN

[2] KANAZAWA UNIV,SCH MED,DEPT ANAT,KANAZAWA,ISHIKAWA 920,JAPAN

来源：

NEUROSCIENCE LETTERS | 1990年 / 117卷 / 03期

关键词：

In situ hybridization; Myelin-specific protein genes; Oligodendrocyte; Severed optic nerve;

D O I：

10.1016/0304-3940(90)90675-Y

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The level and distribution of mRNAs for several myelin-specific proteins were analyzed by in situ hybridization histochemistry in the mouse optic nerves after severance by eye enucleation during the period of active myelination, viz. the 2nd postnatal week. The autoradiographic signals for 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNP)-, myelin basic protein (MBP)- and myelin-associated glycoprotein (MAG)-mRNAs all decreased progressively in the postoperative time course and all the signals fell below the detectable level by the 7th postoperative day. These findings indicate that the levels of genes for all the three major myelin-specific proteins in oligodendrocytes in vivo are down-regulated at the transcriptional level after axonal withdrawal. © 1990.

引用

页码：269 / 274

页数：6

共 15 条

[1] IMPROVED HYBRIDIZATION ASSAYS EMPLOYING TAILED OLIGONUCLEOTIDE PROBES - A DIRECT COMPARISON WITH 5'-END-LABELED OLIGONUCLEOTIDE PROBES AND NICK-TRANSLATED PLASMID PROBES [J].