MOLECULAR-CLONING OF A NEW BOMBESIN RECEPTOR SUBTYPE EXPRESSED IN UTERUS DURING PREGNANCY

被引：129

作者：

GORBULEV, V ^{[1
]}

AKHUNDOVA, A ^{[1
]}

BUCHNER, H ^{[1
]}

FAHRENHOLZ, F ^{[1
]}

机构：

[1] MAX PLANCK INST BIOPHYS, KENNEDYALLEE 70, W-6000 FRANKFURT 70, GERMANY

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1992年 / 208卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1992.tb17201.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The homology screening approach has been used to clone a new member of the guanine-nucleotide-binding-protein-coupled receptor superfamily from guinea pig uterus. The cloned cDNA encodes a 399-amino-acid protein and shows the highest amino acid similarity to members of the bombesin receptor family; 52% and 47% similarity to the gastrin-releasing-peptide (GRP) receptor and the neuromedin-B receptor, respectively. Binding experiments with the stably transfected LLC-PK1 cell line expressing the new receptor protein confirmed the bombesin-like nature of the cloned receptor. The relative order of ligand affinity, GRP = neuromedin C much greater than neuromedin B, suggests that the cloned cDNA represents the GRP subtype rather than the neuromedin-B subtype of bombesin receptors. Northern-blot analysis of mRNA species from several guinea-pig tissues showed that the mRNA for the new bombesin receptor subtype is expressed mainly in uteri of pregnant animals.

引用

页码：405 / 410

页数：6

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