EXPRESSION OF CLONED AROMATIC L-AMINO-ACID DECARBOXYLASE IN XENOPUS-LAEVIS OOCYTES

被引：13

作者：

GUDEHITHLU, KP

DUCHEMIN, AM

SILVIA, CP

NEFF, NH

HADJICONSTANTINOU, M

机构：

[1] OHIO STATE UNIV,COLL MED,DEPT PHARMACOL,COLUMBUS,OH 43210

[2] OHIO STATE UNIV,COLL MED,DEPT PSYCHIAT,COLUMBUS,OH 43210

[3] OHIO STATE UNIV,COLL MED,NEUROSCI PROGRAM,COLUMBUS,OH 43210

来源：

NEUROCHEMISTRY INTERNATIONAL | 1992年 / 21卷 / 02期

关键词：

D O I：

10.1016/0197-0186(92)90158-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sense mRNA coding for bovine adrenal medulla aromatic L-amino acid decarboxylase (AADC) was expressed following microinjection into Xenopus laevis oocytes. The expressed enzyme activity was stereoselective for L-5-hydroxytryptophan and L-DOPA and blocked by NSD-1015 an inhibitor of AADC. Heating the expressed enzyme at 55-degree-C resulted in a parallel loss of activity towards both substrates. Our findings are consistent with the prevailing notion that a single enzyme is able to decarboxylate both substrates in vivo.

引用

页码：275 / 279

页数：5

共 30 条

[1] VARIATIONS IN AROMATIC AMINO-ACID DECARBOXYLASE ACTIVITY TOWARDS DOPA AND 5-HYDROXYTRYPTOPHAN CAUSED BY PH CHANGES AND DENATURATION [J].