EXPRESSION OF CLONED AROMATIC L-AMINO-ACID DECARBOXYLASE IN XENOPUS-LAEVIS OOCYTES

被引：13

作者：

GUDEHITHLU, KP

DUCHEMIN, AM

SILVIA, CP

NEFF, NH

HADJICONSTANTINOU, M

机构：

[1] OHIO STATE UNIV,COLL MED,DEPT PHARMACOL,COLUMBUS,OH 43210

[2] OHIO STATE UNIV,COLL MED,DEPT PSYCHIAT,COLUMBUS,OH 43210

[3] OHIO STATE UNIV,COLL MED,NEUROSCI PROGRAM,COLUMBUS,OH 43210

来源：

NEUROCHEMISTRY INTERNATIONAL | 1992年 / 21卷 / 02期

关键词：

D O I：

10.1016/0197-0186(92)90158-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sense mRNA coding for bovine adrenal medulla aromatic L-amino acid decarboxylase (AADC) was expressed following microinjection into Xenopus laevis oocytes. The expressed enzyme activity was stereoselective for L-5-hydroxytryptophan and L-DOPA and blocked by NSD-1015 an inhibitor of AADC. Heating the expressed enzyme at 55-degree-C resulted in a parallel loss of activity towards both substrates. Our findings are consistent with the prevailing notion that a single enzyme is able to decarboxylate both substrates in vivo.

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页码：275 / 279

页数：5

相关论文

共 30 条

[11] ISOLATION AND CHARACTERIZATION OF A CDNA CLONE ENCODING HUMAN AROMATIC L-AMINO-ACID DECARBOXYLASE [J].

ICHINOSE, H ;

KUROSAWA, Y ;

TITANI, K ;

FUJITA, K ;

NAGATSU, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 164 (03) :1024-1030

[12] DEDUCED AMINO-ACID-SEQUENCE OF BOVINE AROMATIC L-AMINO-ACID DECARBOXYLASE - HOMOLOGY TO OTHER DECARBOXYLASES [J].

KANG, UJ ;

JOH, TH .

MOLECULAR BRAIN RESEARCH, 1990, 8 (01) :83-87

[13] DIFFERENT MESSENGER-RNAS CODE FOR DOPA DECARBOXYLASE IN TISSUES OF NEURONAL AND NONNEURONAL ORIGIN [J].

KRIEGER, M ;

COGE, F ;

GROS, F ;

THIBAULT, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (06) :2161-2165

[14]

LEVITT M, 1965, J PHARMACOL EXP THER, V148, P1

[15] OCCURRENCE AND DISTRIBUTION OF AROMATIC L-AMINO ACID (L-DOPA) DECARBOXYLASE IN HUMAN BRAIN [J].

LLOYD, KG ;

HORNYKIEWICZ, O .

JOURNAL OF NEUROCHEMISTRY, 1972, 19 (06) :1549-+

[16]

LOVENBERG W, 1962, J BIOL CHEM, V237, P89

[17]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[18] NEW AND HIGHLY SENSITIVE VOLTAMMETRIC ASSAY FOR AROMATIC L-AMINO-ACID DECARBOXYLASE ACTIVITY BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

NAGATSU, T ;

YAMAMOTO, T ;

KATO, T .

ANALYTICAL BIOCHEMISTRY, 1979, 100 (01) :160-165

[19] ACCURATE ASSAY OF DOPA DECARBOXYLASE BY PREVENTING NON-ENZYMATIC DECARBOXYLATION OF DOPA [J].

OKUNO, S ;

FUJISAWA, H .

ANALYTICAL BIOCHEMISTRY, 1983, 129 (02) :412-415

[20] 2-PHENYLETHYLAMINE - A MODULATOR OF CATECHOLAMINE TRANSMISSION IN THE MAMMALIAN CENTRAL-NERVOUS-SYSTEM [J].

PATERSON, IA ;

JUORIO, AV ;

BOULTON, AA .

JOURNAL OF NEUROCHEMISTRY, 1990, 55 (06) :1827-1837