BRUGIA-PAHANGI - IDENTIFICATION AND CHARACTERIZATION OF AN AMINOPEPTIDASE ASSOCIATED WITH LARVAL MOLTING

被引：56

作者：

HONG, XQ ^{[1
]}

BOUVIER, J ^{[1
]}

WONG, MM ^{[1
]}

YAMAGATA, GYL ^{[1
]}

MCKERROW, JH ^{[1
]}

机构：

[1] UNIV CALIF DAVIS,DEPT VET MICROBIOL,DAVIS,CA 95616

来源：

EXPERIMENTAL PARASITOLOGY | 1993年 / 76卷 / 02期

关键词：

MOLTING; NEMATODE; BRUGIA; AMINOPEPTIDASE (EC 3.4.11.); METALLOPROTEASE;

D O I：

10.1006/expr.1993.1015

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The third-stage larvae (L3) of the filarid parasite Brugia pahangi were surveyed for protease activity beginning with release from the mosquito vector through molting to the fourth-stage larvae (L4). A metalloaminopeptidase with a substrate preference for phenylalanine was released during the molting process. A screen of aminopeptidase inhibitors identified H-boroPhenylalanine(pinacol) as highly effective, with a K(i) of 1.3 x 10-11 M. This peptidase inhibitor also arrested molting of L3 larvae in vitro at a concentration of 100 μM. © 1994 Academic Press. All rights reserved.

引用

页码：127 / 133

页数：7

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