ISOLATION OF A FAMILY OF CDNA CLONES FROM RICINUS-COMMUNIS L WITH CLOSE HOMOLOGY TO THE HEXOSE CARRIERS

被引:42
作者
WEIG, A [1 ]
FRANZ, J [1 ]
SAUER, N [1 ]
KOMOR, E [1 ]
机构
[1] UNIV REGENSBURG,D-93053 REGENSBURG,GERMANY
关键词
RICINUS; HETEROLOGOUS EXPRESSION; POLYMERASE-CHAIN-REACTION (CLONING); QUANTITATIVE PCR; SUGAR TRANSPORT;
D O I
10.1016/S0176-1617(11)81683-4
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The sugar transport of the so-called group m transport proteins, among them the two so far identified hexose carries from plants, have some strongly conserved sequences in common. Oligonucleotides coding for two conserved regions were constructed to amplify, by polymerase-chain-reaction (PCR), those mRNA species from Ricinus cotyledons that probably code for carrier proteins. Eight different sequences for putative hexose carrier proteins were identified; two of them were completed to full-length clones. Transformation into Saccharomyces cerevisiae showed that one of them definitely codes for an active glucose transport system. The other clone was, though transcribed, not expressed in yeast. The expression frequency was determined by Northern analysis and quantitative PCR. All 8 putative hexose carrier clones were present in only very small amounts in poly(A)-RNA of Ricinus tissues. The identified glucose carrier sequence (Hex3) was predominantely expressed in roots and sink leaves, whereas the other functionally so far unidentified full-length clone (Hex1) was mostly expressed in roots, hypocotyl and source leaf. Both full-length clones showed little or hardly any expression in cotyledons.
引用
收藏
页码:178 / 183
页数:6
相关论文
共 23 条
  • [1] SEQUENCE OF THE LACTOSE PERMEASE GENE
    BUCHEL, DE
    GRONENBORN, B
    MULLERHILL, B
    [J]. NATURE, 1980, 283 (5747) : 541 - 545
  • [2] THE YEAST SNF3-GENE ENCODES A GLUCOSE TRANSPORTER HOMOLOGOUS TO THE MAMMALIAN PROTEIN
    CELENZA, JL
    MARSHALLCARLSON, L
    CARLSON, M
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (07) : 2130 - 2134
  • [3] DAVIS EO, 1987, J BIOL CHEM, V262, P13928
  • [4] AN MF ALPHA-1-SUC2 (ALPHA-FACTOR-INVERTASE) GENE FUSION FOR STUDY OF PROTEIN LOCALIZATION AND GENE-EXPRESSION IN YEAST
    EMR, SD
    SCHEKMAN, R
    FLESSEL, MC
    THORNER, J
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (23): : 7080 - 7084
  • [5] FRIEDEMANN P, 1967, PLANT PHYSIOL, V42, P174
  • [6] GIORGIO N, 1941, MEMORIE SERIE, V5, P113
  • [7] HARPER JF, 1990, J BIOL CHEM, V265, P13601
  • [8] EXPRESSION CLONING AND CDNA SEQUENCING OF THE NA+/GLUCOSE COTRANSPORTER
    HEDIGER, MA
    COADY, MJ
    IKEDA, TS
    WRIGHT, EM
    [J]. NATURE, 1987, 330 (6146) : 379 - 381
  • [9] TRANSFORMATION OF INTACT YEAST-CELLS TREATED WITH ALKALI CATIONS
    ITO, H
    FUKUDA, Y
    MURATA, K
    KIMURA, A
    [J]. JOURNAL OF BACTERIOLOGY, 1983, 153 (01) : 163 - 168
  • [10] SUCROSE TRANSPORT INTO THE PHLOEM OF RICINUS-COMMUNIS L SEEDLINGS AS MEASURED BY THE ANALYSIS OF SIEVE-TUBE SAP
    KALLARACKAL, J
    ORLICH, G
    SCHOBERT, C
    KOMOR, E
    [J]. PLANTA, 1989, 177 (03) : 327 - 335