INCREASES IN [CA2+](I) MEDIATED BY THE 92.5-KDA PUTATIVE CELL-MEMBRANE RECEPTOR FOR HCMV GP86

被引:19
作者
KEAY, S
BALDWIN, BR
SMITH, MW
WASSERMAN, SS
GOLDMAN, WF
机构
[1] UNIV MARYLAND, SCH MED, DEPT MED, DEPT INFECT DIS, BALTIMORE, MD 21201 USA
[2] UNIV MARYLAND, SCH MED, DEPT MED, DEPT GEOG MED, BALTIMORE, MD 21201 USA
[3] UNIV MARYLAND, SCH MED, DEPT PATHOL, BALTIMORE, MD 21201 USA
[4] UNIV MARYLAND, SCH MED, DEPT PHYSIOL, BALTIMORE, MD 21201 USA
[5] DEPT VET AFFAIRS MED CTR, CTR GERIATR RES EDUC & CLIN, BALTIMORE, MD 21201 USA
[6] DEPT VET AFFAIRS MED CTR, RES SERV, BALTIMORE, MD 21201 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 1995年 / 269卷 / 01期
关键词
CYTOMEGALOVIRUS; TRANSMEMBRANE SIGNALING; INTRACELLULAR CALCIUM ION CONCENTRATION;
D O I
10.1152/ajpcell.1995.269.1.C11
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We determined that changes in intracellular Ca2+ concentration ([Ca2+](i)) occur in human fibroblasts within the first hour of human cytomegalovirus (HCMV) infection when viral adsorption and fusion take place, and we investigated whether such changes also occur in response to monoclonal anti-idiotype antibodies (MAb(2)) that mimic HCMV gp86 and bind to a 92.5-kDa putative cell membrane receptor for gp86. Digitized image analysis of fura 2-loaded human embryonic lung fibroblasts indicated specific transient increases in [Ca2+](i) beginning in some cells within the first 5 min of incubation with cross-linked MAb(2) (70-750 nM, P < 0.01), which were similar in timing and intracellular distribution to those induced by HCMV. A primary source of Ca2+ appeared to be intracellular Ca2+ stores, since prior depletion of these stores with 30 nM thapsigargin inhibited the response (91.7 +/- 8.6%, P < 0.01); influx of Ca2+ from the extracellular medium was apparently necessary to maintain the intracellular Ca2+ stores. Transient increases in inositol trisphosphate (IP3) occurred in response to MAb(2) (up to 3,329 +/- 84%, P < 0.001) or HCMV (92.8 +/- 19%, P < 0.01) during this same time period. These data suggest that the 92.5-kDa receptor for HCMV gp86 mediates an increase in IP3 and subsequent release of Ca2+ from intracellular stores.
引用
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页码:C11 / C21
页数:11
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