RNA-DIRECTED DE-NOVO METHYLATION OF GENOMIC SEQUENCES IN PLANTS

被引：659

作者：

WASSENEGGER, M

HEIMES, S

RIEDEL, L

SANGER, HL

机构：

[1] Max-Planck-Institut für Biochemie Abteilung Viroidforschung

来源：

CELL | 1994年 / 76卷 / 03期

关键词：

D O I：

10.1016/0092-8674(94)90119-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

One monomeric and three oligomeric potato spindle tuber viroid (PSTVd) cDNA units were introduced into the tobacco genome via the Agrobacterium-mediated leaf-disc transformation. Southern analysis of the integrates revealed that only their PSTVd-specific sequences become fully methylated, whereas the flanking T-DNA and the genomic plant DNA remain unaltered. Viroid cDNA methylation could only be observed after autonomous viroid RNA-RNA replication had taken place in these plants. These findings demonstrate that a mechanism of de novo methylation of genes might exist that can be induced and targeted in a sequence-specific manner by their own mRNA.

引用

页码：567 / 576

页数：10

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[1] ACCELERATION OF NUCLEIC-ACID HYBRIDIZATION RATE BY POLYETHYLENE-GLYCOL [J].