KINETICS OF CYCLOSPORINE-A-INDUCED INHIBITION OF SUCCINATE COENZYME-Q DEHYDROGENASE IN RAT RENAL CORTICAL MITOCHONDRIA

被引：8

作者：

LEMMI, CAE

PELIKAN, PCD

GEESAMAN, B

SEAMON, E

KOYLE, M

RAJFER, J

机构：

[1] UNIV CALIF LOS ANGELES,LOS ANGELES CTY HARBOR MED CTR,DEPT MED,DIV NEPHROL,TORRANCE,CA 90509

[2] ST JOHNS HEART INST,SANTA MONICA,CA 90404

[3] UNIV CALIF LOS ANGELES,LOS ANGELES CTY HARBOR MED CTR,DEPT SURG,DIV UROL,TORRANCE,CA 90509

[4] UNIV CALIF LOS ANGELES,LOS ANGELES CTY HARBOR MED CTR,DEPT MED,DIV CARDIOL,TORRANCE,CA 90509

来源：

BIOCHEMICAL MEDICINE AND METABOLIC BIOLOGY | 1990年 / 43卷 / 03期

关键词：

D O I：

10.1016/0885-4505(90)90027-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The kinetic mechanism of succinate-coenzyme Q dehydrogenase (Complex II) inhibition by cyclosporine A (CS) on rat renal cortical mitochondria was investigated. CS showed two modes of inhibition of Complex II of the mitochondrial electron transport system: (a) a mixed linear noncompetitive inhibition of resting succinate-limited and ADP-stimulated respirations suggesting that CS binds to Complex II at a different site than the substrate, affecting the dissociation constant for the enzyme-substrate complex and (b) a competitive inhibition of the DNP-stimulated electron transport system suggesting competition with the oxidized form of a component of Complex II. CS action to renal mitochondrial Complex II limits its function, an effect which may be related to CS nephrotoxicity. © 1990.

引用

页码：214 / 225

页数：12

共 23 条

[1] INFLUENCE OF CYCLOSPORINE-A TREATMENT ON INTRACELLULAR MEMBRANES OF HEPATOCYTES [J].