NMR LIPID PROFILES OF CELLS, TISSUES AND BODY-FLUIDS .1. 1D AND 2D PROTON NMR OF LIPIDS FROM RAT-LIVER

A combination of established lipid extraction procedures and high-resolution 1D and 2D NMR spectroscopic methods has been used to analyse qualitatively and quantitatively the lipid composition of rat liver. The major lipids present in each structural class were identified and quantified. Considerable information on the composition of the different classes of structural and unsaturated fatty acid chains were obtained for the intact lipids without resort to hydrolysis. Significantly, the relative amounts of cholesterol, glycerophospholipids, ether lipids, sphingolipids and glycerides were shown to be in agreement with previous determinations by high-performance liquid and other chromatographic procedures.