INVESTIGATIONS ON VINYLENE CARBONATE .6. IMMOBILIZATION OF ALKALINE-PHOSPHATASE ONTO POLY(VINYLENE CARBONATE)-JEFFAMINE(R) HYDROGEL BEADS

Hydrogel-like polymers with reactive cyclic carbonate (CCA) groups have been obtained by the cross-linking reaction of poly(vinylene carbonate) (PVCA) with Jeffamines(R). By this reaction, microspheric hydrogel beads with a high water content and a high concentration of reactive CCA groups were prepared. The beads were used as a matrix for the immobilization of the enzyme alkaline phosphatase (ALP) and showed a considerable capacity to couple ALP and a reasonable retention of activity for the immobilized ALP, depending on the reaction conditions. The immobilized ALP exhibited a better thermal stability than did native ALP and a high residual activity was found after repeated use.