MASS-SPECTROMETRY IN THE DIAGNOSIS OF STEROID-RELATED DISORDERS AND IN HYPERTENSION RESEARCH

被引：224

作者：

SHACKLETON, CHL

机构：

[1] Children's Hospital Oakland Research Institute, Oakland, CA 94609

来源：

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1993年 / 45卷 / 1-3期

关键词：

D O I：

10.1016/0960-0760(93)90132-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of GC/MS and microbore HPLC/electrospray mass spectrometry for clinical studies in hypertension and mineralocorticoid research is described. In particular, an automated GC/MS method allows nearly quantitative measurements of metabolites of major steroids of adrenal, placental and gonadal origin. This method is able to distinguish almost all steroid related disorders. Electrospray mass spectrometry in conjunction with microbore HPLC is the latest mass spectrometry technique applicable to biochemical investigations. It offers high sensitivity (15 pg) for measurement of intact steroid conjugates and related compounds.

引用

页码：127 / 140

页数：14

共 33 条

[1]

CHU MD, 1982, J BIOL CHEM, V257, P2218

[2]

CHUNG BC, IN PRESS STEROIDS

[3] STABLE ISOTOPE-DILUTION METHOD USING THERMOSPRAY LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY FOR QUANTIFICATION OF DAILY CORTISOL PRODUCTION IN HUMANS [J].