DIRECT OBSERVATION OF THE IRON-BINDING SITES IN A FERRITIN

被引：90

作者：

HEMPSTEAD, PD ^{[1
]}

HUDSON, AJ ^{[1
]}

ARTYMIUK, PJ ^{[1
]}

ANDREWS, SC ^{[1
]}

BANFIELD, MJ ^{[1
]}

GUEST, JR ^{[1
]}

HARRISON, PM ^{[1
]}

机构：

[1] UNIV SHEFFIELD, KREBS INST, DEPT MOLEC BIOL & BIOTECHNOL, SHEFFIELD S10 2UH, ENGLAND

来源：

FEBS LETTERS | 1994年 / 350卷 / 2-3期

关键词：

FERRITIN; IRON-BINDING SITE; BINUCLEAR FE COMPLEX; IRON MINERALIZATION;

D O I：

10.1016/0014-5793(94)00781-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

X-Ray analysis of the ferritin of Escherichia coli (Ec FTN) and of Ec-FTN crystals soaked in (NH4)(2)Fe(SO4)(2) has revealed the presence of three iron-binding sites per subunit. Two of these form a di-iron site in the centre of the subunit as has been proposed for the 'ferroxidase centres' of human ferritin H chains. This di-iron site, lying within the 4-alpha-helix bundle, resemble those of ribonucleotide reductase, methane monoxygenase and haemerythrin. The third iron is bound by ligands unique to Ec-FTN on the inner surface of the protein shell. It is speculated that this state may represent the nucleation centre of a novel type of Fe(III) cluster, recently observed in Ec-FTN.

引用

页码：258 / 262

页数：5

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