GROWTH AND COPPER RESISTANCE OF RECOMBINANT SACCHAROMYCES-CEREVISIAE CONTAINING A METALLOTHIONEIN GENE

被引：4

作者：

HAN, NS

SEO, JH

CHUNG, YC

机构：

[1] SEOUL NATL UNIV, DEPT FOOD SCI & TECHNOL, SUWON 441744, SOUTH KOREA

[2] KOREA INST SCI & TECHNOL, SEOUL 130650, SOUTH KOREA

来源：

BIOTECHNOLOGY LETTERS | 1992年 / 14卷 / 01期

关键词：

D O I：

10.1007/BF01030905

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Resistance to copper's toxicity in S. cerevisiae is mediated by a copper binding protein, metallothionein (MT). Overexpression of MT in the recombinant yeast containing multiple copies of the MT gene on plasmid leads to a four-fold increase in the critical copper resistance level and an eight-fold enhancement of specific growth rate in a 2mM CuSO4 medium compared with the parental host strain. The recombinant yeast grown in a 5mM CuSO4 medium was found to accumulate 24.1mg of Cu per g of dry cell mass.

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页码：7 / 10

页数：4

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