EX-VIVO PERFUSION WITH ANTICOAGULATED BLOOD DECREASES ISCHEMIA-REPERFUSION INJURY

被引：13

作者：

LI, XL ^{[1
]}

COOLEY, BC ^{[1
]}

GOULD, JS ^{[1
]}

机构：

[1] MED COLL WISCONSIN,DEPT ORTHOPAED SURG,8700 W WISCONSIN AVE,MILWAUKEE,WI 53226

来源：

JOURNAL OF HAND SURGERY-AMERICAN VOLUME | 1993年 / 18A卷 / 04期

关键词：

D O I：

10.1016/0363-5023(93)90306-N

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

摘要：

Rat muscle flaps (cutaneous maximus) were subjected to 4 hours of room-temperature ischemia followed by 44 hours of cold (4-degrees-C) ischemia before transplantation. Experimental flaps underwent a 15-minute ex vivo perfusion with heparinized-citrated blood between the warm and cold ischemic treatments; control flaps were not perfused. After 1 or 48 hours of recirculation, muscle dehydrogenase activity was found to be higher in the perfused flaps, indicating better muscle viability. Lipid peroxidation was lower in the perfused flaps, indicating a reduction in free radical generation. Histologic assessment revealed that perfused flaps underwent less injury than unperfused flaps. The findings of this study indicate that replacement of standing blood with anticoagulated blood, with the use of a brief ex vivo perfusion protocol, has a significant protective effect against ischemic injury. The experimental design mimics a realistic point of intervention and presents a paradigm for the clinical treatment of amputated extremities.

引用

页码：629 / 634

页数：6

共 38 条

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