CARBOHYDRATES AS A TOOL FOR ORIENTED IMMOBILIZATION OF ANTIGENS AND ANTIBODIES

被引：27

作者：

TURKOVA, J

PETKOV, L

SAJDOK, J

KAS, J

BENES, MJ

机构：

[1] PRAGUE INST CHEM TECHNOL, CS-16628 PRAGUE 6, CZECHOSLOVAKIA

[2] CZECHOSLOVAK ACAD SCI, INST MACROMOLEC CHEM, CS-16206 PRAGUE 6, CZECHOSLOVAKIA

来源：

JOURNAL OF CHROMATOGRAPHY | 1990年 / 500卷

关键词：

D O I：

10.1016/S0021-9673(00)96093-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A biospecific sorbent for the isolation of ovalbumin antibodies was prepared by coupling of ovalbumin via its periodate-oxidized carbohydrate moiety to bead cellulose modified with adipic acid dihydrazide. The anti-ovalbumin IgG fraction isolated on this sorbent from immune rabbit serum contained only antibodies against protein determinants of ovalbumin. Thus, when these IgG were immobilized through their carbohydrate moieties to cellulose beads it became possible to prepare a biospecific sorbent for concanavalin A by oriented adsorption of ovalbumin. Ovalbumin was specifically adsorbed via its protein moiety and its carbohydrate part remained free for interaction with concanavalin A. © 1990.

引用

页码：585 / 593

页数：9

共 17 条

[1] A SIMPLE METHOD FOR THE ESTIMATION OF AMINO-GROUPS ON INSOLUBLE MATRIX BEADS [J].