STUDIES ON PROPHENOLOXIDASE ACTIVATION IN THE MOSQUITO AEDES-AEGYPTI L

被引：67

作者：

ASHIDA, M ^{[1
]}

KINOSHITA, K ^{[1
]}

BREY, PT ^{[1
]}

机构：

[1] INST PASTEUR, UNITE LUTTE BIOL CONTRE INSECTES, 25 RUE DR ROUX, F-75724 PARIS 15, FRANCE

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1990年 / 188卷 / 03期

关键词：

D O I：

10.1111/j.1432-1033.1990.tb15429.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study, the first of its kind in a mosquito vector species, demonstrates the feasibility of studying prophenoloxidase activation in an insect containing not more than a few microliters of hemolymph. Mosquito phenoloxidase was found to be in an inactive proenzyme form, prophenoloxidase. Mosquito prophenoloxidase required bivalent cation for its activation; Ca2+ was found to be the most efficient for activation. Concomitant amidase activity was also observed prior to phenoloxidase activity. Through Western blotting, using a cross‐reactive silkworm anti‐prophenoloxidase antibody, our results strongly suggest that mosquito prophenoloxidase activation resulted from limited proteolysis. Protease inhibitor studies reinforced this contention showing the involvement of (a) serine protease(s) with trypsin‐like activity in the activation of mosquito prophenoloxidase. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：507 / 515

页数：9

共 44 条

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