RAPID SYNTHESIS OF OLIGODEOXYRIBONUCLEOTIDES .3. EFFECT OF ADDED CARBOXYLATE ION ON THE EFFICIENCY OF INTERNUCLEOTIDE BOND FORMATION - SOLID-PHASE SYNTHESIS OF THE DODECANUCLEOTIDE, D(PT-A-A-C-T-G-C-T-C-A-C-T)

In solid-phase oligodeoxyribonucleotide synthesis the addition of benzoic acid to internucleotide phosphodiester bond-forming reactions lowers coupling yields by a far greater amount than anticipated on a simple nucleotide-carboxy-late molar ratio. High coupling yields were consistently obtained in synthesis of the dodecanucleotide, d(pT-A-A-C-T-G-C-T-C-A-C-T), using rigorously purified nucleotide starting materials. © 1979 Information Retrieval Limited.