ULTRAVIOLET-LASER DESORPTION IONIZATION MASS-SPECTROMETRY OF PROTEINS ABOVE 100000 DALTONS BY PULSED ION EXTRACTION TIME-OF-FLIGHT ANALYSIS

被引：149

作者：

SPENGLER, B ^{[1
]}

COTTER, RJ ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV,SCH MED,DEPT PHARMACOL & MOLEC SCI,MIDDLE ATLANTIC MASS SPECTROMETRY FACIL,BALTIMORE,MD 21205

来源：

ANALYTICAL CHEMISTRY | 1990年 / 62卷 / 08期

关键词：

D O I：

10.1021/ac00207a004

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

UV laser desorption mass spectra of large proteins (up to 116 kDa) have been obtained on a Wiley-McLaren type time-offlight Instrument with pulsed ion extraction. Intense ion signals could be observed following delay times of several microseconds prior to ion acceleration and extraction. Initial kinetic energy distributions of ions could be determined from the drift time between the probe tip and the ion optical axis and were found to be about 1 eV, in contrast to measurements by retarding potential techniques at the detector site. © 1990, American Chemical Society. All rights reserved.

引用

页码：793 / 796

页数：4

共 22 条

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