ION-EXCHANGE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ANALYSIS OF OLIGODEOXYRIBONUCLEOTIDE PHOSPHOROTHIOATES

被引：39

作者：

METELEV, V ^{[1
]}

AGRAWAL, S ^{[1
]}

机构：

[1] WORCESTER FDN EXPTL BIOL INC,SHREWSBURY,MA 01545

来源：

ANALYTICAL BIOCHEMISTRY | 1992年 / 200卷 / 02期

关键词：

D O I：

10.1016/0003-2697(92)90476-N

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Oligodeoxynucleotide-containing phosphorothioate backbones have been used to regulate viral as well as cellular gene expression. The studies carried out in tissue culture have shown promising results on the use of oligonucleotide phosphorothioates as antiviral agents and, at present, study is underway to develop these oligonucleotide analogues as chemotherapeutic agents. To analyze and purify oligonucleotide analogues, high-performance liquid chromatography using weak anion exchange column has been described. The separation of oligonucleotide phosphorothioate is found to be length dependent. © 1992.

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页码：342 / 346

页数：5

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