COMPARISON OF CNS HOMING PATTERN AMONG MURINE TH CELL-LINES RESPONSIVE TO MYELIN BASIC-PROTEIN

A myelin basic protein (MBP)-reactive T(H) cell line capable of inducing experimental allergic encephalomyelitis (EAE), and a MBP-reactive T(H) cell clone that does not cause EAE were labeled with a fluorescent vital dye, and transferred into naive syngeneic SJL/J mice. Animals were killed before, the appearance of symptoms (3 and 4 days post-injection). Sections obtained from the spleen, spinal cord and brain of both groups of animals were examined by fluorescence microscopy to localize labeled T(H) cells. At all time points examined, the spleens of both groups contained innumerable labeled cells. The spinal cords and brains of animals that had received EAE-causing cells had a basal level of 20 labeled cells/cm2 at 3 days; this number increased rapidly to 150 cellS/CM2 in the spinal cord at 4 days. Perivascular infiltrates and small foci of astrogliosis were already apparent in this group 3 days after injection. The spinal cords and brains of animals that had received the non-EAE-causing T(H) cells contained 50 labeled cells/cm2 at 3 days. The density of these transferred cells, as compared to that of the EAE-causing cells, suggested that they have an unaltered CNS-homing capability. However, by 4 days, the number of non-EAE-causing labeled cells had returned to near basal level. Our findings suggest that discrimination between disease and non-disease causing MBP-responsive T(H) cells occurs within the first 3 days following transfer, requires the presence in the CNS of a limited number of T(H) cells, and depends on yet unidentified T(H) cell factor(s).