SEQUENCE-DEPENDENT STRUCTURAL VARIATIONS OF DNA REVEALED BY CHEMICAL LIGATION

被引：12

作者：

DOLINNAYA, NG

MERENKOVA, IN

SHABAROVA, ZA

机构：

[1] Chemistry Department, M.V.Lomonosov Moscow State University, 119899

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 1994年 / 13卷 / 10期

关键词：

D O I：

10.1080/15257779408013215

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The comparative study of nick-sealing efficiency under the action of BrCN or water-soluble carbodiimide was carried out for 14 dinucleotide combinations in double helix. The difference between the lowest (17%, GpG) and the highest (94%, CpT) coupling yields was found to be more than five fold, both condensing reagents showing a similar sequence-specific trend. A strong correlation observed between coupling yields at different dinucleotide combinations and (31)p NMR parameters supports the idea that variations in chemical ligation efficiency arise from sequence-specific modulations of the helix geometry and confirms close similarity of the intrinsic fine structure of intact and nicked DNAs.

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页码：2169 / 2183

页数：15

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