SEQUENCE-INDEPENDENT AMPLIFICATION AND LABELING OF YEAST ARTIFICIAL CHROMOSOMES FOR FLUORESCENCE IN-SITU HYBRIDIZATION

被引：49

作者：

BOHLANDER, SK ^{[1
]}

ESPINOSA, R ^{[1
]}

FERNALD, AA ^{[1
]}

ROWLEY, JD ^{[1
]}

LEBEAU, MM ^{[1
]}

DIAZ, MO ^{[1
]}

机构：

[1] UNIV CHICAGO,HEMATOL ONCOL SECT,CHICAGO,IL 60637

来源：

CYTOGENETICS AND CELL GENETICS | 1994年 / 65卷 / 1-2期

关键词：

D O I：

10.1159/000133612

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have developed a method that allows reliable construction of high quality FISH probes from yeast artificial chromosomes (YACs) based on the separation of YACs by pulsed-field gel electrophoresis and a rapid sequence-independent amplification procedure (SIA). These probes can be used to localize YACs on metaphase chromosomes and also with high efficiency, in interphase nuclei.

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页码：108 / 110

页数：3

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