CALRETININ IS EXPRESSED IN THE LEYDIG-CELLS OF RAT TESTIS

被引：47

作者：

STRAUSS, KI

ISAACS, KR

HA, QN

JACOBOWITZ, DM

机构：

[1] NIMH, Laboratory of Clinical Science, Bethesda, MD 20892, Bldg. 10

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1994年 / 1219卷 / 02期

关键词：

CALRETININ; IMMUNOCYTOCHEMISTRY; RNASE PROTECTION ASSAY; WESTERN BLOT; IN SITU HYBRIDIZATION; (RAT TESTIS); (LEYDIG CELL);

D O I：

10.1016/0167-4781(94)90069-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Calretinin, a highly evolutionarily conserved E-F hand calcium binding protein, is expressed predominantly in neurons, with a few exceptions. The function of calretinin is not known. We demonstrate the expression of calretinin mRNA and protein in rat testes. Immunocytochemistry and in situ hybridization reveal that calretinin expression in testis is localized to the interstitial Leydig cells. Western blot and ribonuclease protection analyses show that calretinin protein and mRNA in testis is the same as that expressed in brain. It is suggested that calretinin may play a role in the production of testosterone.

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页码：435 / 440

页数：6

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