ISOLATION AND IDENTIFICATION OF 4-HYDROXYRETINOIC AND 4-OXORETINOIC ACID - INVITRO METABOLITES OF ALL-TRANS-RETINOIC ACID IN HAMSTER TRACHEA AND LIVER

被引：128

作者：

FROLIK, CA ^{[1
]}

ROBERTS, AB ^{[1
]}

TAVELA, TE ^{[1
]}

ROLLER, PP ^{[1
]}

NEWTON, DL ^{[1
]}

SPORN, MB ^{[1
]}

机构：

[1] NCI, BETHESDA, MD 20014 USA

来源：

BIOCHEMISTRY | 1979年 / 18卷 / 10期

关键词：

D O I：

10.1021/bi00577a039

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Incubation of [3H]retinoic acid in the presence of hamster liver 10,000 g supernatant produces several metabolites that are more polar than the parent compound. Two of these metabolites are identical with synthetic all-trans-4-hydroxyretinoic acid and all-trans-4-oxoretinoic acid both in UV absorption and mass spectral characteristics and in migration rates on 2 different reverse-phase, high-pressure liquid chromatographic (HPLC) systems. The metabolites produced in a cell-free liver incubation reaction also migrate on a HPLC column together with metabolites isolated from a tracheal organ culture system. Both the metabolites and the synthetic standards show less biological activity than the parent all-trans-retinoic acid in a tracheal organ culture assay.

引用

页码：2092 / 2097

页数：6

共 35 条

[1] ALPHA-RETINYL BETA-RETINYL ACETATE REVERSE METAPLASIAS OF VITAMIN-A-DEFICIENCY IN HAMSTER TRACHEA IN ORGAN-CULTURE [J].