DETERMINATION OF 7-DEHYDROCHOLESTEROL IN HUMAN SKIN BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：12

作者：

MOODY, JP

HUMPHRIES, CA

ALLAN, SM

PATERSON, CR

机构：

[1] Department of Biochemical Medicine, Ninewells Hospital, Medical School, Dundee

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1990年 / 530卷 / 01期

关键词：

D O I：

10.1016/S0378-4347(00)82298-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A two-stage chromatographic procedure has been devised for the measurement of 7-dehydrocholesterol in human skin. Extracts containing ergosterol as internal standard underwent preparative chromatography on a Spherisorb S5W column using hexane-1% isopropanol as solvent, and an eluted fraction was analysed with an Ultrasphere 5-μm ODS column with methanol-tetrahydrofuran-17.5 mM KH2PO4 (95:1:4, v/v) as solvent and using an amperometric detector at 1.7 V. 7-Dehydrocholesterol could be reliably assayed in human skin samples as small as 5 mm in diameter. In hospital patients skin 7-dehydrocholesterol concentrations ranged from 12 to 81 μg/g dry weight. © 1990.

引用

页码：19 / 27

页数：9

共 12 条

[1] MEASUREMENT OF BLOOD-LEVELS OF 7-DEHYDROCHOLESTEROL IN MAN BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].