CHARACTERIZATION OF RAT-BRAIN NCAM MESSENGER-RNA USING DNA OLIGONUCLEOTIDE PROBES

被引：21

作者：

ANDERSSON, AM

GAARDSVOLL, H

GILADI, E

DAHL, B

BOCK, E

机构：

[1] UNIV COPENHAGEN,HC ORSTED INST,CHEM LAB 2,DK-1168 COPENHAGEN,DENMARK

[2] OREGON REG PRIMATE RES CTR,DIV NEUROSCI,BEAVERTON,OR 97006

来源：

FEBS LETTERS | 1990年 / 263卷 / 02期

关键词：

Neural cell adhesion molecule; Northern blotting; Oligonucleotide;

D O I：

10.1016/0014-5793(90)81420-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A number of different isoforms of the neural cell adhesion molecule (NCAM) have been identified. The difference between these is due to alternative splicing of a single NCAM gene. In rat brain NCAM mRNAs with sizes of 7.4, 6.7, 5.2, 4.3 and 2.9 kb have been reported. We have synthesized six DNA oligonucleotides, that hybridize to different exons in the NCAM gene. Furthermore we have constructed three oligonucleotides, that exclusively hybridize to mRNAs lacking certain exons, by letting them consist of sequences adjacent to both sides of the splice sites. By means of these probes we have characterized the five NCAM mRNAs in rat brain. © 1990.

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页码：385 / 388

页数：4

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